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Intracytoplasmic Sperm Injection (ICSI)<< back to IVF Table of Contents Intracytoplasmic Sperm Injection "(ICSI") is a new modality in the treatment of severe male factor infertility. ICSI is indicated where the partner has less than 10 million motile sperm per milliliter, where there is abnormal sperm morphology, sperm penetration, acrosomal reaction, or repeated failure to fertilize your or a donor's eggs in prior IVF attempts. ICSI might also be indicated in cases of egg defects, which limit or inhibit spontaneous fertilization in vitro. ICSI is a technique of gamete micromanipulation in which a single sperm is captured in a microscopic glass pipette and meticulously injected into the cytoplasm of a single egg. Harvested, mature eggs are selected to undergo this delicate procedure. Although we are able to use very poor semen samples, the laboratory still requires several normal appearing motile sperm for injection (at least one for each good egg).
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